Journal: Applied and Environmental Microbiology

Article Title: Biofilm Sampling for Detection of Cryptosporidium Oocysts in a Southeastern Pennsylvania Watershed

doi: 10.1128/AEM.01399-20

Figure Lengend Snippet: Seasonal oocyst detection by IFA from filter and submersible slide biofilm samples at the WTP. (A) Astronomical seasons; (B) meteorological seasons. n is the total number of samples collected.

Article Snippet: Previous work in this watershed ( 31 ) included phylogenetic analyses that identified C. parvum and C. hominis as well as the cervine, skunk, chipmunk I, duck, snake, rat, muskrat, deer mouse III, squirrel, and W19 genotypes; these genotypes indicate both human and wildlife sources of Cryptosporidium in the surface water, which makes sense for this urban/suburban mixed-use watershed. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Sample identifier Location Sample type(s) Tributary A Suburban (impacted by WWTP discharge) Biofilms (rocks, submersible slides) Tributary B Urban (impacted by defective sewer laterals) Biofilms (rocks, submersible slides), filters WTP Urban Biofilms (submersible slides), filters Open in a separate window Sample locations and types table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Sample type Location(s) Date range Kit IMS IFA Biofilms (benthic rock, submersible slide) Tributary A September 2010–February 2011 Aureon Merifluor Biofilms (benthic rock) Tributary B October 2014–March 2015 Dynabeads Crypt-a-Glo Biofilms (submersible slide), filtered water WTP, tributary B July 2013–August 2014 Virusys Crypt-a-Glo Open in a separate window Overview of study methods The general methodologies for Cryptosporidium oocyst detection were similar for each study (sampling, immunomagnetic separation [IMS], and immunofluorescence assay [IFA]), but the method details varied because the studies were conducted at different times over several years.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Biofilm Sampling for Detection of Cryptosporidium Oocysts in a Southeastern Pennsylvania Watershed

doi: 10.1128/AEM.01399-20

Figure Lengend Snippet: Seasonal oocyst detection by IFA from filter and submersible slide biofilm samples at tributary B. Note that no samples were collected in summer 1. (A) Astronomical seasons; (B) meteorological seasons. n is the total number of samples collected.

Article Snippet: Previous work in this watershed ( 31 ) included phylogenetic analyses that identified C. parvum and C. hominis as well as the cervine, skunk, chipmunk I, duck, snake, rat, muskrat, deer mouse III, squirrel, and W19 genotypes; these genotypes indicate both human and wildlife sources of Cryptosporidium in the surface water, which makes sense for this urban/suburban mixed-use watershed. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Sample identifier Location Sample type(s) Tributary A Suburban (impacted by WWTP discharge) Biofilms (rocks, submersible slides) Tributary B Urban (impacted by defective sewer laterals) Biofilms (rocks, submersible slides), filters WTP Urban Biofilms (submersible slides), filters Open in a separate window Sample locations and types table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Sample type Location(s) Date range Kit IMS IFA Biofilms (benthic rock, submersible slide) Tributary A September 2010–February 2011 Aureon Merifluor Biofilms (benthic rock) Tributary B October 2014–March 2015 Dynabeads Crypt-a-Glo Biofilms (submersible slide), filtered water WTP, tributary B July 2013–August 2014 Virusys Crypt-a-Glo Open in a separate window Overview of study methods The general methodologies for Cryptosporidium oocyst detection were similar for each study (sampling, immunomagnetic separation [IMS], and immunofluorescence assay [IFA]), but the method details varied because the studies were conducted at different times over several years.

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Biofilm Sampling for Detection of Cryptosporidium Oocysts in a Southeastern Pennsylvania Watershed

doi: 10.1128/AEM.01399-20

Figure Lengend Snippet: Overview of study methods

Article Snippet: Previous work in this watershed ( 31 ) included phylogenetic analyses that identified C. parvum and C. hominis as well as the cervine, skunk, chipmunk I, duck, snake, rat, muskrat, deer mouse III, squirrel, and W19 genotypes; these genotypes indicate both human and wildlife sources of Cryptosporidium in the surface water, which makes sense for this urban/suburban mixed-use watershed. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Sample identifier Location Sample type(s) Tributary A Suburban (impacted by WWTP discharge) Biofilms (rocks, submersible slides) Tributary B Urban (impacted by defective sewer laterals) Biofilms (rocks, submersible slides), filters WTP Urban Biofilms (submersible slides), filters Open in a separate window Sample locations and types table ft1 table-wrap mode="anchored" t5 TABLE 2 caption a7 Sample type Location(s) Date range Kit IMS IFA Biofilms (benthic rock, submersible slide) Tributary A September 2010–February 2011 Aureon Merifluor Biofilms (benthic rock) Tributary B October 2014–March 2015 Dynabeads Crypt-a-Glo Biofilms (submersible slide), filtered water WTP, tributary B July 2013–August 2014 Virusys Crypt-a-Glo Open in a separate window Overview of study methods The general methodologies for Cryptosporidium oocyst detection were similar for each study (sampling, immunomagnetic separation [IMS], and immunofluorescence assay [IFA]), but the method details varied because the studies were conducted at different times over several years.

Techniques:

Journal: Sudanese Journal of Paediatrics

Article Title: COVID-19 and lessons learned from the pandemic wave of meningococcal meningitis (1985–1990)

doi: 10.24911/SJP.106-1587809750

Figure Lengend Snippet: ELISA test kit (Meningitis EIA-Test, Karo-Bio Diagnostics, Stockholm, Sweden) with four plastic tubes coated with rabbit antisera against Haemophilus influenzae type b. Variable dilutions of cerebrospinal fluid (CSF) samples from which H. influenzae type b was isolated were added to the tubes. The development of yellow-orange colour observed by the naked eye is considered a positive reaction, as seen in the two tubes in the middle.

Article Snippet: The importance of rapid antigen tests was highlighted during the current COVID-19 pandemic to guide patient management and infection control strategies, and were considered essential for slowing down the pandemic [ 8 ]. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1. caption a7 ELISA test kit (Meningitis EIA-Test, Karo-Bio Diagnostics, Stockholm, Sweden) with four plastic tubes coated with rabbit antisera against Haemophilus influenzae type b.

Techniques: Enzyme-linked Immunosorbent Assay, Isolation

Journal: The Journal of Infectious Diseases

Article Title: Inflammation and Metabolism of Influenza-Stimulated Peripheral Blood Mononuclear Cells From Adults With Obesity Following Bariatric Surgery

doi: 10.1093/infdis/jiac345

Figure Lengend Snippet: The percentage of T-helper 2 (Th2) and select B-cell populations are impaired with obesity. Immune cell populations in resting and A/California/07/2009pdm H1N1 influenza–stimulated peripheral blood mononuclear cells (PBMCs) from lean (n = 10–13), presurgery (n = 13–14), and postsurgery (n = 12–13) participants. A, viSNE plots of PBMCs stained with the Human Immune Monitoring Kit and analyzed on the Fluidigm Helios 2.0 using mass cytometry by time of flight. B, Populations with significant changes based on clustering and biaxial gating (Supplementary Figure 1) for activated CD4 T cells, CD4 Th2 cells, central memory CD4 T cells, and terminal effector CD4 T cells. C, Significant changes in switched memory B cells, nonswitched memory B cells, and transitional B cells. Data are mean ± standard deviation. Comparisons were made by nonpaired Wilcoxon and paired Wilcoxon tests with Benjamini–Hochberg correction to limit the false discovery rate: +P < .10, *P < .05, ***P < .001. Abbreviations: CM, central memory; DC, dendritic cell; NK, natural killer; PBMC, peripheral blood mononuclear cell; TCR, T cell receptor; TE, terminal effector; Th2, T-helper 2.

Article Snippet: A , viSNE plots of PBMCs stained with the Human Immune Monitoring Kit and analyzed on the Fluidigm Helios 2.0 using mass cytometry by time of flight.

Techniques: Staining, Mass Cytometry, Standard Deviation